mouse monoclonal neuronal nitric oxide synthase nnos antibody  (Santa Cruz Biotechnology)

Journal: Translational Andrology and Urology

Article Title: The influence of smoking exposure and cessation on penile hemodynamics and corporal tissue in a rat model

doi: 10.21037/tau.2019.12.45

Figure Lengend Snippet: Neuronal nitric oxide synthase (nNOS) in corpora cavernosa. Representative images from rats with exposure only to room air for 12 weeks (N), smoke exposure for a full 12 weeks followed by immediate erectile function testing (S), smoke exposure for 12 weeks followed by 4 weeks of room air only exposure before erectile function testing (Q4W) and smoke exposure for 12 weeks followed by 8 weeks of room air only exposure before erectile function testing (Q8W). nNOS-positive fibers are strained brown with nNOS antibody. Positive staining for nNOS was less intense in the S group than in the N and Q8W groups. (Scale bars: left, 500 µm; right, 100 µm).

Article Snippet: Briefly, tissue sections were stained with Masson’s trichrome staining, mouse anti-8-hydroxy-2'-deoxyguanosine (8-OHdG, 1:50; JaICA, Nikken Seil Co., Japan), mouse anti- neuronal nitric oxide synthase (nNOS, 1:100; BD Transduction Laboratories, Franklin Lakes, NJ, USA), and terminal deoxynucleotidyl transferasemediated deoxyuridine triphosphate nick-end labeling (TUNEL, Roche Diagnostics Corporation, Indianapolis, IN, USA) using standard techniques.

Techniques: Staining

Journal: Translational Andrology and Urology

Article Title: The influence of smoking exposure and cessation on penile hemodynamics and corporal tissue in a rat model

doi: 10.21037/tau.2019.12.45

Figure Lengend Snippet: Western blot for endothelial nitric oxide synthase (eNOS). Representative images from rats with exposure only to room air for 12 weeks (N), smoke exposure for a full 12 weeks followed by immediate erectile function testing (S), smoke exposure for 12 weeks followed by 4 weeks of room air only exposure before erectile function testing (Q4W) and smoke exposure for 12 weeks followed by 8 weeks of room air only exposure before erectile function testing (Q8W). Mean eNOS was significantly lower in the S group compared with N and Q8W groups; significant differences between the N and Q4W groups were also noted. (*, P<0.05; **, P<0.01).

Article Snippet: Briefly, tissue sections were stained with Masson’s trichrome staining, mouse anti-8-hydroxy-2'-deoxyguanosine (8-OHdG, 1:50; JaICA, Nikken Seil Co., Japan), mouse anti- neuronal nitric oxide synthase (nNOS, 1:100; BD Transduction Laboratories, Franklin Lakes, NJ, USA), and terminal deoxynucleotidyl transferasemediated deoxyuridine triphosphate nick-end labeling (TUNEL, Roche Diagnostics Corporation, Indianapolis, IN, USA) using standard techniques.

Techniques: Western Blot